Equipment
NEPAF is one of the most advanced proteomic facilities in the UK - We operate multiple Mass Spectromers, including the revolutionary Bruker Daltonics MaXis(TM) TOF Mass Spectrometer. This system gives ultra high mass resolution over a broad mass range. We also have the Thermo Fisher Scientific LTQ Orbitrap XL(TM) Mass Spectrometer with Electron Transfer Dissociation (ETD) for identification of post-translational modifications to proteins.
Equipment available at NEPAF includes:
- Bruker Ultraflex II MALDI TOF/TOF (particularly useful for the identification of proteins from 2DE gels)
- Applied Biosytems 4800 MALDI TOF/TOF (also suitable for Maldi imaging)
- Bruker HCT Ultra Proteome Discovery system (ETD) with Dionex Ultimate 3000 nano-HPLC
- Applied Biosystems Qtrap 4000 with Dionex Ultimate 3000 nano-HPLC
- ThermoScience Orbitrap XL (ETD) with Dionex Ultimate 3000 nano-HPLC
- Bruker maXis with Dionex Ultimate 3000 nano-HPLC
- Standalone Dionex Ultimate 3000 nano-HPLC system with micro fraction collector, capable of performing 2D-LC analyses;
- Zeptosens reverse protein array platform;
- GE BiaCore X100
- GE Typhoon Trio+ gel scanner
- GE Akta explorer FPLC
- ProGenesis SameSpots and ProGenesis LCMS software; OpenMS software, in house Matrixscience Mascot Server.
